Generation of influenza transfectants using purified recombinant nucleocapsid protein
Identifieur interne : 001B06 ( Main/Exploration ); précédent : 001B05; suivant : 001B07Generation of influenza transfectants using purified recombinant nucleocapsid protein
Auteurs : Michael W. Shaw [États-Unis] ; Irina V. Kiseleva [Russie] ; Andrei Y. Egorov [Russie] ; Mark L. Hemphill [États-Unis] ; Xiyan Xu [États-Unis]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1998.
English descriptors
- KwdEn :
- Teeft :
- Assay, Baculovirus, Clone, Culture supernates, Elsevier science, Enami, Helper virus, Klimov, Mdck cells, Nucleocapsid, Palese, Polymerase, Reassortant, Recombinant, Recombinant baculovirus, Restriction analysis, Transcription, Transcription reactions, Transfectant viruses, Transfectants, Transfected, Transfection, Transfection assays, Virion, Virion polymerase proteins, Virol, Virological methods, Virus transfectants.
Abstract
Abstract: Affinity-purified type A influenza virus nucleocapsid protein expressed by a recombinant baculovirus vector was used in in vitro RNA transcription reactions to create RNP complexes containing a synthetic influenza A virus NS gene. When used in transfection assays, the baculovirus-expressed NP was shown to be biologically active allowing the efficient isolation of transfectant viruses containing the artificially-introduced NS gene. The results demonstrate that NP is the only virion protein necessary in the reconstituted RNP complexes used for transfection thus eliminating the need for purified RNP complexes containing active polymerase.
Url:
DOI: 10.1016/S0166-0934(98)00114-1
Affiliations:
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Egorov</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Virology, Institute for Experimental Medicine, Academy of Medical Science, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="L Hemphill, Mark" sort="L Hemphill, Mark" uniqKey="L Hemphill M" first="Mark" last="L. Hemphill">Mark L. Hemphill</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta GA 30333</wicri:regionArea><wicri:noRegion>Atlanta GA 30333</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Xiyan" sort="Xu, Xiyan" uniqKey="Xu X" first="Xiyan" last="Xu">Xiyan Xu</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta GA 30333</wicri:regionArea><wicri:noRegion>Atlanta GA 30333</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">76</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="149">149</biblScope><biblScope unit="page" to="151">151</biblScope></imprint><idno type="ISSN">0166-0934</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Baculovirus</term><term>Polymerase</term><term>Ribonucleoprotein</term><term>Transfection</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Baculovirus</term><term>Clone</term><term>Culture supernates</term><term>Elsevier science</term><term>Enami</term><term>Helper virus</term><term>Klimov</term><term>Mdck cells</term><term>Nucleocapsid</term><term>Palese</term><term>Polymerase</term><term>Reassortant</term><term>Recombinant</term><term>Recombinant baculovirus</term><term>Restriction analysis</term><term>Transcription</term><term>Transcription reactions</term><term>Transfectant viruses</term><term>Transfectants</term><term>Transfected</term><term>Transfection</term><term>Transfection assays</term><term>Virion</term><term>Virion polymerase proteins</term><term>Virol</term><term>Virological methods</term><term>Virus transfectants</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Affinity-purified type A influenza virus nucleocapsid protein expressed by a recombinant baculovirus vector was used in in vitro RNA transcription reactions to create RNP complexes containing a synthetic influenza A virus NS gene. When used in transfection assays, the baculovirus-expressed NP was shown to be biologically active allowing the efficient isolation of transfectant viruses containing the artificially-introduced NS gene. The results demonstrate that NP is the only virion protein necessary in the reconstituted RNP complexes used for transfection thus eliminating the need for purified RNP complexes containing active polymerase.</div></front></TEI><affiliations><list><country><li>Russie</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="W Shaw, Michael" sort="W Shaw, Michael" uniqKey="W Shaw M" first="Michael" last="W. Shaw">Michael W. Shaw</name></noRegion><name sortKey="L Hemphill, Mark" sort="L Hemphill, Mark" uniqKey="L Hemphill M" first="Mark" last="L. Hemphill">Mark L. Hemphill</name><name sortKey="Xu, Xiyan" sort="Xu, Xiyan" uniqKey="Xu X" first="Xiyan" last="Xu">Xiyan Xu</name></country><country name="Russie"><noRegion><name sortKey="V Kiseleva, Irina" sort="V Kiseleva, Irina" uniqKey="V Kiseleva I" first="Irina" last="V. Kiseleva">Irina V. Kiseleva</name></noRegion><name sortKey="Y Egorov, Andrei" sort="Y Egorov, Andrei" uniqKey="Y Egorov A" first="Andrei" last="Y. Egorov">Andrei Y. Egorov</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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